Tris is a very important biological buffer, commonly used as a combo with hydroxymethyl aminomethane, keeping pH at 7.0-9.1 stably. It is sensitive to temperature and concentration. The pH value of Tris based aqueous solution is about 10.5. Hydrochloric  is usually added to adjust the pH value to the desired value. EDTA is usually added to Tris hydrochloric  buffer to make "TE buffer".

TE buffer was used for DNA stabilization and storage. When the  solution adjusting the pH value is replaced by acetic, the "TAE buffer" is obtained(Tris / acetate / EDTA), while the solution adjusting the pH value is replaced by byboric, the "TBE buffer" is obtained(Tris / borate / EDTA).

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